ytrB [2020-08-25 12:57:13]

ABC transporter (ATP-binding protein)

Locus: BSU_30450

Isoelectric point: 5.51

Molecular weight: 33.67 kDa

Protein length: 292 aa

Gene length: 879 bp

Function: unknown

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Unknown ABC transporters
• Lifestyles → Coping with stress → Cold stress proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,117,976 → 3,118,854

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 2-227) (according to UniProt)

Structure

• 1VPL (from Thermotoga maritima, 31% identity)

Localization

• cell membrane (via YtrC-YtrD) PubMed

Expression and Regulation

Operons

• Genes: ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • YtrA: repression, PubMed, in YtrA regulon

  Regulation

  • expressed early in the stationary phase PubMed

Biological materials

Mutant

• GP3188 Δ(ytrB), available in Jörg Stülke's lab
• GP2646 Δ(ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF)::ermC, available in Jörg Stülke's lab
• MGNA-A131 (ytrB::erm), available at the NBRP B. subtilis, Japan
• BKE30450 (ΔytrB::erm trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC, downstream forward: _UP4_AGATGGTAGACCGAGGTCTC
• BKK30450 (ΔytrB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC, downstream forward: _UP4_AGATGGTAGACCGAGGTCTC

References

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